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Calculate the degree of ionization of acetic acid in solution 3. The degree of ionization of an acid = [H3O+]/[HA] where [H3O+] is the equilibrium concentration and [HA] is the original concentration of of the acid . [H3O+] can be determined from the measured pH. Solution 3. 10mL of 0.1 M HC2H3O2 + 90.0 mL of H2O Measured pH 3.51. To determine the [H3O+] account for the dilution effect of adding 90.0 mL of water , and use M1V1=M2V2