Fed-State Metabolism and the Effects of Insulin Video Lessons

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Problem: Two cell cultures, culture A and culture B, containing a combination of epithelial cells and adipocytes, were cultured in a medium containing 5% serum, containing all of the enzymes and signaling molecules found in the blood plasma needed to keep the cells aliveĀ in vitro. In addition, culture A was supplemented with a constant supply of glucose, while culture B was supplemented with glucose every eight hours. These two different cultures were maintained for one week under these conditions and observed daily for changes in metabolic rate and cell size.How will the size of the adipocyte differ between culture A and culture B after one week?

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Fed State: Adipose tissues also convert excess glucose into triglycerides

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Problem Details

Two cell cultures, culture A and culture B, containing a combination of epithelial cells and adipocytes, were cultured in a medium containing 5% serum, containing all of the enzymes and signaling molecules found in the blood plasma needed to keep the cells alive in vitro. In addition, culture A was supplemented with a constant supply of glucose, while culture B was supplemented with glucose every eight hours. These two different cultures were maintained for one week under these conditions and observed daily for changes in metabolic rate and cell size.

How will the size of the adipocyte differ between culture A and culture B after one week?

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