RNA Interference

Which of the following is likely to contain the most acrylamide? a. raw potato left in the skin b. fried potato c. baked potato d. boiled potato

Members of the Argonaute protein family play key roles in RNA interference pathways (RNAi). RNAi lowers gene expression in response to double-stranded RNA (dsRNA) whether endogenous (e.g. pre-miRNA) or exogenous (e.g. viral genomes). The RNA process is initiated when Dicer, an RNase IlI family enzyme, cleaves dsRNA into short duplexes (~21 base pairs), each strand possessing a single-stranded, two-nucleotide overhang on its 3'–end and a phosphate on its 5'–terminus. These dsRNAs (siRNAs or miRNAs) are then loaded onto Argonaute (Ago).Once engaged by Ago, one of the siRNA or miRNA strands, the passenger strand, is cut and discarded. The remaining single-stranded RNA (ssRNA) is termed the guide strand. Ago acts as the scaffold for the recognition of complementary target sequences by the guide strand. Additionally, as part of the RNA-induced silencing complex (RISC), Ago can catalyze the cleavage (slicing) of target mRNAs.Identify the domains of argonaute labeled 1-3a. PAZ domain (1), Middle domain (2), N-terminal domain (3) b. PIWI domain (1), Middle domain (2), PAZ domain (3)c. Middle domain (1), PIWI domain (2), N-terminal domain (3)d. PAZ domain (1), PIWI domain (2), N-terminal domain (3)Which Argonatue domain contains the active sitea. PAZ domainb. N-terminal domainc. Middle domaind. PIWI domain

You are a plant geneticist and wish to alter strawberries using the same technique as was used for the Innate Potato. Which of the following do you use? a. RNA interference b. DNA manufacture c. RNA recombination d. DNA rearrangement

You are interested in trying an Innate Potato. Where are you most likely to find one? a. You will have to plant the seeds yourself. b. Your local farmers market. c. Your local grocery store. d. McDonald's

siRNAs and miRNAs are produced by the a. cutting and processing of double-stranded RNA by Slicer enzymes. b. cleavage of pre-mRNA in the nucleus. c. cleavage of functional mRNA within the cytoplasm. d. cutting and processing of double-stranded RNA by Dicer enzymes. e. cleavage of RISCs by endonucleases.

Long noncoding RNAs that partially overlap protein-coding genes and are transcribed in the opposite direction are best classified as ________.A. intronic lncRNA genesB. piwi interacting RNAsC. antisense lncRNA genesD. bidirectional lncRNA genesE. microRNAs