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Problem: DNAse I is an enzyme that cleaves DNA accessible to it.When chromatin is remodeled during transcription, regulatory DNA becomes accessible to cleavage by DNAse I. In contrast, regulatory DNA of genes not actively transcribed is not cleaved by DNAse I.Results show that DNA from the older brother is completely sensitive to DNAase I digestion while DNA from the affected child is partially sensitive to DNAse I digestion.Regulatory DNAPromoterTranscribed RegionExpression?1.Affected childAffected childAffected childNo2.Older brotherOlder brotherOlder brotherYes3.Older brotherOlder brotherAffected childYes4.Affected childOlder brotherOlder brotherNo5.Older brotherAffected childOlder brotherYesWhich of the three hypotheses do these results support? A. The results support the hypothesis that the defect in the child is due to a mutation in the transcribed region that causes mRNA instability.B. The results support the hypothesis that the defect in the affected child is due to regulatory DNA that will not remodel.C. The results support the hypothesis that the defect in the affected child is due to a defective promoter.

FREE Expert Solution

Given:


Regulatory DNA

Promoter

Transcribed Region






Expression?

1.

Affected child

Affected child

Affected child

No

2.

Older brother

Older brother

Older brother

Yes

3.

Older brother

Older brother

Affected child

Yes

4.

Affected child

Older brother

Older brother

No

5.

Older brother

Affected child

Older brother

Yes

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Problem Details

DNAse I is an enzyme that cleaves DNA accessible to it.

When chromatin is remodeled during transcription, regulatory DNA becomes accessible to cleavage by DNAse I. In contrast, regulatory DNA of genes not actively transcribed is not cleaved by DNAse I.

Results show that DNA from the older brother is completely sensitive to DNAase I digestion while DNA from the affected child is partially sensitive to DNAse I digestion.


Regulatory DNA

Promoter

Transcribed Region






Expression?

1.

Affected child

Affected child

Affected child

No

2.

Older brother

Older brother

Older brother

Yes

3.

Older brother

Older brother

Affected child

Yes

4.

Affected child

Older brother

Older brother

No

5.

Older brother

Affected child

Older brother

Yes


Which of the three hypotheses do these results support? 

A. The results support the hypothesis that the defect in the child is due to a mutation in the transcribed region that causes mRNA instability.

B. The results support the hypothesis that the defect in the affected child is due to regulatory DNA that will not remodel.

C. The results support the hypothesis that the defect in the affected child is due to a defective promoter.

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