Antisense RNA: single-strand DNA that hybridizes with mRNA, blocking translation
Several examples of antisense RNA regulating translation in bacterial cells have been discovered. If you wanted to design a construct to artificially control translation of a bacterial gene with antisense RNA, what type of sequences should the antisense RNA contain?
A. A sequence complementary to the operator of an operon.
B. A sequence complementary to a 5'–UTR.
C. A sequence complementary to the Shine-Dalgarno site of the mRNA.
D. Choices A and B are correct.
E. Choices B and C are correct.
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