Overview of DNA Replication Video Lessons

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Problem: A universal feature of nucleic acid polymerases is the use of two metal cations to catalyze the addition of new nucleotides to a growing chain. In the T7 DNA polymerase active site, two magnesium ions are positioned to facilitate the polymerization reaction. One cation interacts with the 3’ OH on the primer strand, whereas the other cation stabilizes the negative charges on all three phosphates of the incoming nucleotide. Identify the different components in this SN2 reaction. Identify the different components in this SN2 reaction. A. The nucleophile is the alpha phosphorous atom (orange). The leaving group is the diphosphate (dark blue). B. The nucleophile is the ionized form of the OH group (red). The leaving group is the diphosphate (dark blue). C. The nucleophile is the diphosphate (dark blue). The leaving group is the OH group (red). D. The nucleophile is the ionized form of the OH group (red). The leaving group is the alpha phosphorus atom (orange).

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A universal feature of nucleic acid polymerases is the use of two metal cations to catalyze the addition of new nucleotides to a growing chain. In the T7 DNA polymerase active site, two magnesium ions are positioned to facilitate the polymerization reaction. One cation interacts with the 3’ OH on the primer strand, whereas the other cation stabilizes the negative charges on all three phosphates of the incoming nucleotide. Identify the different components in this SN2 reaction. Identify the different components in this SN2 reaction. 

A. The nucleophile is the alpha phosphorous atom (orange). The leaving group is the diphosphate (dark blue). 

B. The nucleophile is the ionized form of the OH group (red). The leaving group is the diphosphate (dark blue). 

C. The nucleophile is the diphosphate (dark blue). The leaving group is the OH group (red). 

D. The nucleophile is the ionized form of the OH group (red). The leaving group is the alpha phosphorus atom (orange).

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