Lambda Bacteriophage and Life Cycle Regulation Video Lessons

Video Thumbnail

Concept

Problem: The mechanistic details of DNA synthesis in higher organisms is not completely understood, in part because so many molecules are involved in the process. The bacteriophage T7 DNA replication complex is a good model system for the study of the mechanism of DNA synthesis because it consists of relatively few proteins.Which of the following describes the origins of these two components? A. Both components are encoded in the phage genome. B. Both components are encoded in the host (E. coli) genome. C. The polymerase proper is encoded in the phage genome, whereas the processivity factor is encoded in the host (E. coli) genome.D. The polymerase proper is encoded in the host genome (E. coli), whereas the processivity factor is encoded in the phage genome.In which domain is the bottom of the DNA-binding cleft? What type of secondary structure lines the bottom of the cleft? A. The bottom of the cleft is in the fingers domain. It is lined with alpha helices. B. The bottom of the cleft is in the thumb domain. It is lined with both alpha helices and a beta sheet. C.The bottom of the cleft is in the palm domain. It is lined with a beta sheet. D.The bottom of the cleft is in the palm domain. It is lined with alpha helices. E. The bottom of the cleft is in the fingers domain. It is lined with a beta sheet.

FREE Expert Solution

T7 DNA Polymerase:


View Complete Written Solution
Problem Details

The mechanistic details of DNA synthesis in higher organisms is not completely understood, in part because so many molecules are involved in the process. The bacteriophage T7 DNA replication complex is a good model system for the study of the mechanism of DNA synthesis because it consists of relatively few proteins.

Which of the following describes the origins of these two components? 

A. Both components are encoded in the phage genome. 

B. Both components are encoded in the host (E. coli) genome. 

C. The polymerase proper is encoded in the phage genome, whereas the processivity factor is encoded in the host (E. coli) genome.

D. The polymerase proper is encoded in the host genome (E. coli), whereas the processivity factor is encoded in the phage genome.


In which domain is the bottom of the DNA-binding cleft? What type of secondary structure lines the bottom of the cleft? 

A. The bottom of the cleft is in the fingers domain. It is lined with alpha helices. 

B. The bottom of the cleft is in the thumb domain. It is lined with both alpha helices and a beta sheet. 

C.The bottom of the cleft is in the palm domain. It is lined with a beta sheet. 

D.The bottom of the cleft is in the palm domain. It is lined with alpha helices. 

E. The bottom of the cleft is in the fingers domain. It is lined with a beta sheet.

Frequently Asked Questions

What scientific concept do you need to know in order to solve this problem?

Our tutors have indicated that to solve this problem you will need to apply the Lambda Bacteriophage and Life Cycle Regulation concept. You can view video lessons to learn Lambda Bacteriophage and Life Cycle Regulation. Or if you need more Lambda Bacteriophage and Life Cycle Regulation practice, you can also practice Lambda Bacteriophage and Life Cycle Regulation practice problems.

What professor is this problem relevant for?

Based on our data, we think this problem is relevant for Professor Lee's class at UAB.