End-Replication Problem: Eukaryotes have no place to produce the RNA primer needed to start the last Okazaki fragment at the very tip of a linear DNA molecule
A problem known as the end-replication problem (telomere problem) exists in eukaryotic chromosomes wherein the chromosomes shorten with each round of DNA replication. Select the statements that best explain why the end-replication problem exists in eukaryotic chromosomes (select all statements that apply)
1) The RNA primer is removed in a 3' to 5' direction.
2) DNA polymerase requires a primer for DNA synthesis.
3) DNA ligase links the 5' OH group of one fragment to the 3' phosphate group of an adjacent fragment.
4) The lagging strand is synthesized from the 3' end to the 5' end.
5) DNA polymerase synthesizes DNA from the phosphate end to the hydroxyl end.