Computer programmers, working with molecular geneticists, have developed computer programs that can identify genes within long stretches of DNA sequences. Imagine that you are working with a computer programmer on such a project. On the basis of what you know about the process of transcription, what sequences should be used to identify the beginning and end of a prokaryotic gene with the use of this computer program? Select all that apply.
A) Sequences with close similarity to the -35 and -10 sequences upstream of start site.
B) A stop codon indicates the last transcribed nucleotide.
C) The start codon (AUG) indicates the first transcribed base.
D) Sequences with close similarity to the -25 TATA box are found near the beginning of a gene.
E) Short, inverted repeats followed by at least seven T nucleotides indicating potential intrinsic termination sites.
F) At least five inverted repeats indicate the last transcribed nucleotide that indicates intrinsic termination site.
E) Sequences with 10 to 20 repeats of A and T nucleotides mark the beginning of most genes.