Transcription in Prokaryotes Video Lessons

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Problem: Computer programmers, working with molecular geneticists, have developed computer programs that can identify genes within long stretches of DNA sequences. Imagine that you are working with a computer programmer on such a project. On the basis of what you know about the process of transcription, what sequences should be used to identify the beginning and end of a prokaryotic gene with the use of this computer program? Select all that apply. A) Sequences with close similarity to the -35 and -10 sequences upstream of start site. B) A stop codon indicates the last transcribed nucleotide. C) The start codon (AUG) indicates the first transcribed base.D) Sequences with close similarity to the -25 TATA box are found near the beginning of a gene. E) Short, inverted repeats followed by at least seven T nucleotides indicating potential intrinsic termination sites. F) At least five inverted repeats indicate the last transcribed nucleotide that indicates intrinsic termination site. E) Sequences with 10 to 20 repeats of A and T nucleotides mark the beginning of most genes.

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Computer programmers, working with molecular geneticists, have developed computer programs that can identify genes within long stretches of DNA sequences. Imagine that you are working with a computer programmer on such a project. On the basis of what you know about the process of transcription, what sequences should be used to identify the beginning and end of a prokaryotic gene with the use of this computer program? Select all that apply. 


A) Sequences with close similarity to the -35 and -10 sequences upstream of start site. 

B) A stop codon indicates the last transcribed nucleotide. 

C) The start codon (AUG) indicates the first transcribed base.

D) Sequences with close similarity to the -25 TATA box are found near the beginning of a gene. 

E) Short, inverted repeats followed by at least seven T nucleotides indicating potential intrinsic termination sites. 

F) At least five inverted repeats indicate the last transcribed nucleotide that indicates intrinsic termination site. 

E) Sequences with 10 to 20 repeats of A and T nucleotides mark the beginning of most genes.

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Based on our data, we think this problem is relevant for Professor Hartl & Hopkins' class at HARVARD.