Genetic Cloning Video Lessons

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Problem: What is important in cloning the GFP cDNA into the vector? a. The GFP cDNA is inserted after the target protein cDNA. b. The GFP cDNA has its own promoter. c. The GFP cDNA is able to show fluorescence. d. The GFP cDNA is in the same reading frame as the target protein.

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GFP Fusion: involves expressing green fluorescent protein to non-native cells

: mostly used in fluorescent microscopy and determining gene expression levels

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Problem Details

What is important in cloning the GFP cDNA into the vector? 

a. The GFP cDNA is inserted after the target protein cDNA. 

b. The GFP cDNA has its own promoter. 

c. The GFP cDNA is able to show fluorescence. 

d. The GFP cDNA is in the same reading frame as the target protein.

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