Methods for Analyzing DNA Video Lessons

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Problem: Screening the plasmid library. The final step of the cloning procedure is to screen the plasmid library for clones that carry the vgp gene, shown in red below. To accomplish the screening, researchers synthesize a single-stranded DNA probe using vgp mRNA as a template. What will be the sequence of nucleotides in the probe? Drag the labels to their appropriate locations in the probe. Labels can be used once, more than once, or not at all.

FREE Expert Solution

Given: 

vgp DNA template:  3'–TACTTAAG...GTCACACT–5'

vgp mRNA:               5'–AUGAAUUC...CAGUGUGA–3'

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Problem Details
Screening the plasmid library. The final step of the cloning procedure is to screen the plasmid library for clones that carry the vgp gene, shown in red below. 

To accomplish the screening, researchers synthesize a single-stranded DNA probe using vgp mRNA as a template. What will be the sequence of nucleotides in the probe? 

Drag the labels to their appropriate locations in the probe. Labels can be used once, more than once, or not at all.




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