Genetic Cloning Video Lessons

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Problem: Order the events that occur in one cycle of the polymerase chain reaction (PCR). The reaction mixture contains four copies of a particular DNA sequence. The reaction mixture contains eight copies of the DNA sequence.• The reaction mixture is heated to 94°C.• The double-stranded DNA is denatured to form single-stranded DNA.• Taq polymerase synthesizes a new DNA strand.• The temperature of the reaction mixture is lowered to allow the primers to anneal to the DNA template.• The reaction mixture is heated to 72°C.

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Polymerase Chain Reaction: method of amplifying DNA; usually performed using Taq polymerase

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Problem Details

Order the events that occur in one cycle of the polymerase chain reaction (PCR). 

The reaction mixture contains four copies of a particular DNA sequence. 







The reaction mixture contains eight copies of the DNA sequence.


• The reaction mixture is heated to 94°C.

• The double-stranded DNA is denatured to form single-stranded DNA.

Taq polymerase synthesizes a new DNA strand.

• The temperature of the reaction mixture is lowered to allow the primers to anneal to the DNA template.

• The reaction mixture is heated to 72°C.

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