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Problem: Which of the following is true about restriction endonucleases?A. They make a blunt cut on the two DNA strands so that there are no single-strand regions. B. They make staggered cuts on the DNA so that single-strand ends are formed that can be used to insert foreign DNA cut with the same enzyme.C. Some make a blunt cut on the two DNA strands so that there are no single-strand regions and some make staggered cuts on the DNA so that single-strand ends are formed that can be used to insert foreign DNA cut with the same enzyme.D. Depending on the incubation conditions, the same enzyme can either make a blunt cut on the two DNA strands so that there are no single-strand regions OR make staggered cuts on the DNA so that single-strand ends are formed that can be used to insert foreign DNA cut with the same enzyme.

FREE Expert Solution

Restriction Enzymes: cleaves DNA at specific palindromic sequences

: produces either sticky ends (with overhangs) or blunt ends (without overhangs)

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Problem Details

Which of the following is true about restriction endonucleases?

A. They make a blunt cut on the two DNA strands so that there are no single-strand regions. 

B. They make staggered cuts on the DNA so that single-strand ends are formed that can be used to insert foreign DNA cut with the same enzyme.

C. Some make a blunt cut on the two DNA strands so that there are no single-strand regions and some make staggered cuts on the DNA so that single-strand ends are formed that can be used to insert foreign DNA cut with the same enzyme.

D. Depending on the incubation conditions, the same enzyme can either make a blunt cut on the two DNA strands so that there are no single-strand regions OR make staggered cuts on the DNA so that single-strand ends are formed that can be used to insert foreign DNA cut with the same enzyme.

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