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Problem: X-Gal is included in the growth medium on which cells transformed with bacterial plasmids are grown. The reason X-Gal is included is to _______.  a) eliminate bacteria that do not contain recombinant plasmid b) eliminate bacteria that do not contain plasmid DNA c) minimize the chances that a vector will re-circularize without incorporating a fragment of foreign DNA d) identify bacteria that contain a recombinant plasmid.

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X-Gal: galactose linked to a substituted indole; used to screen for bacteria containing recombinant plasmids

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Problem Details

X-Gal is included in the growth medium on which cells transformed with bacterial plasmids are grown. The reason X-Gal is included is to _______.  

a) eliminate bacteria that do not contain recombinant plasmid 

b) eliminate bacteria that do not contain plasmid DNA 

c) minimize the chances that a vector will re-circularize without incorporating a fragment of foreign DNA 

d) identify bacteria that contain a recombinant plasmid.

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