Induced Mutations Video Lessons

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Problem: You recently discovered four new chemical mutagens (#1 - #4), but you do not know what type(s) of mutations these chemicals induce. You exposed E. coli to each chemical individually and observed mutant phenotypes. You then exposed this bacteria to a second known mutagen to observe if the mutations from chemicals #1 - #4 could be reversed. Your data is presented below.Mutations Induced by Mutations Reversed by Chemical EMS Acridine Orange 5-Bromouracil Mutations Induced ByMutations Reversed ByChemicalEMSAcridine Orange5-Bromouracil#1NoYesNo#2YesNoYes#3YesYesYes#4NoNoNoWhat type(s) of mutations are likely produced by chemical #3? A. transversions B. transitions C. frameshifts What type(s) of mutations are likely produced by chemical #4? A. transversions B. transitions C. frameshifts

FREE Expert Solution

Types of mutations: includes transversions, transitions, and frameshifts


Known mutagens: 

• EMS (ethyl methanesulfonate) → alkylating agent → transition mutation (G–C to A–T)

• Acridine Orange → intercalating agent → frameshift mutation

• 5-bromouracil → base analog → transition mutation (G–C to A–T)

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Problem Details

You recently discovered four new chemical mutagens (#1 - #4), but you do not know what type(s) of mutations these chemicals induce. You exposed E. coli to each chemical individually and observed mutant phenotypes. You then exposed this bacteria to a second known mutagen to observe if the mutations from chemicals #1 - #4 could be reversed. Your data is presented below.

Mutations Induced by Mutations Reversed by Chemical EMS Acridine Orange 5-Bromouracil 

Mutations Induced By

Mutations Reversed By

Chemical

EMS

Acridine Orange

5-Bromouracil

#1

No

Yes

No

#2

Yes

No

Yes

#3

Yes

Yes

Yes

#4

No

No

No


What type(s) of mutations are likely produced by chemical #3? 

A. transversions 

B. transitions 

C. frameshifts 


What type(s) of mutations are likely produced by chemical #4? 

A. transversions 

B. transitions 

C. frameshifts

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