A critical reaction in the production of energy to do work or drive chemical reactions in biological systems is the hydrolysis of adenosine triphosphate, ATP, to adenosine diphosphate, ADP, as described by
ATP (aq) + H2O (l) → ADP (aq) + HPO 42- (aq)
for which ΔG°rxn = -30.5 kJ/mol at 37.0 °C and pH 7.0. Calculate the value of ΔG rxn (kJ/mol) in a biological cell in which [ATP] = 5.0 mM, [ADP] = 0.80 mM, and [HPO42-] = 5.0 mM.
Is the hydrolysis of ATP spontaneous under these conditions?
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