# Problem: From the Beer-Lambert Law, A=ebcWhere A is absorbance, e is the molar absorbtivity, b is the path length of the sample - that is, the path length of the cuvette in which the sample is contained, and c is the concentration of the compound in solution.If someone measured a certain absorbance from a solution with a known concentration from the spectrometer, and then measured another solution of the same species at the same wavelength in the same spectrometer, is it possible to calculate the concentration of the second substance (which is currently an unknown)?

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We are asked to determine if it's possible to determine the concentration of a certain substance where its absorbance is known and given that a certain absorbance from another substance with a known concentration from the spectrometer is knownBoth measurements were measured under the same wavelength.

79% (303 ratings)
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From the Beer-Lambert Law,

A=ebc

Where A is absorbance, e is the molar absorbtivity, b is the path length of the sample - that is, the path length of the cuvette in which the sample is contained, and c is the concentration of the compound in solution.

If someone measured a certain absorbance from a solution with a known concentration from the spectrometer, and then measured another solution of the same species at the same wavelength in the same spectrometer, is it possible to calculate the concentration of the second substance (which is currently an unknown)?