Problem: A student finds a solution of blue dye #2 (another food dye we are not using in this lab) in a 1.0 cm cuvette has a maximum absorbance of 0.572 at 613 nm. The molar extinction coefficient, E, of blue #2 at the same wavelength is 1.21 x 104 M-1 cm-1. Calculate the concentration of this solution.

FREE Expert Solution

The absorbance of a solution is related to its concentration by the Beer-Lambert law.

A = εbc

Where,

A = absorption

ε = molar absorptivity

b = path length of solution

c = molar concentration


We have the following data for solution A:

A = 0.572

b = 1.0 cm

ε = 1.21 x 104 M-1 cm-1

c = concentration


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Problem Details

A student finds a solution of blue dye #2 (another food dye we are not using in this lab) in a 1.0 cm cuvette has a maximum absorbance of 0.572 at 613 nm. The molar extinction coefficient, E, of blue #2 at the same wavelength is 1.21 x 104 M-1 cm-1. Calculate the concentration of this solution.

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