The absorbance of a solution is related to its concentration by the Beer-Lambert law.
A = absorption
ε = molar absorptivity
b = path length of solution
c = molar concentration
We have the following data for solution A:
A = 0.572
b = 1.0 cm
ε = 1.21 x 104 M-1 cm-1
c = concentration
A student finds a solution of blue dye #2 (another food dye we are not using in this lab) in a 1.0 cm cuvette has a maximum absorbance of 0.572 at 613 nm. The molar extinction coefficient, E, of blue #2 at the same wavelength is 1.21 x 104 M-1 cm-1. Calculate the concentration of this solution.
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