Problem: If a biochemist wishes to prepare a buffer that will be effective at a pH of 3.0 (at 25°C) for studying peptide degradation, what will be the best choice for the acid component? (a) Oxalic acid (H2C2O4), Ka = 5.9 x 10 -2 (b) Hydrofluoric acid (HF), Ka = 6.6 x 10 -4 (c) Pyridinium ion (HC5H5N+ ), Ka = 5.6 x 10 -6 (d) Hypochlorous acid (HClO), Ka = 3.0 x 10 -8 (e) Hydrocyanic acid (HCN), Ka = 6.17 x 10 -10 

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If a biochemist wishes to prepare a buffer that will be effective at a pH of 3.0 (at 25°C) for studying peptide degradation, what will be the best choice for the acid component?

(a) Oxalic acid (H2C2O4), Ka = 5.9 x 10 -2

(b) Hydrofluoric acid (HF), Ka = 6.6 x 10 -4

(c) Pyridinium ion (HC5H5N+ ), Ka = 5.6 x 10 -6

(d) Hypochlorous acid (HClO), Ka = 3.0 x 10 -8

(e) Hydrocyanic acid (HCN), Ka = 6.17 x 10 -10 

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