DNA Cloning Video Lessons

Video Thumbnail

Concept

Problem: Below is a diagram of the DNA product of a PCR amplification of the region of the human genome containing p53. The white region represents the coding region of the gene. The letters above the DNA represent the locations of specific restriction enzyme cut sites. The numbers below each restriction site represent the number of kilobases (kb) from the first restriction site (at 0 kb). Each of the restriction enzymes creates unique sticky ends. The plasmid into which you want to clone the p53 gene is shown below.After cutting the PCR product with HindIII and NotI, how many pieces of DNA will you have?

FREE Expert Solution

PCR Product: 

View Complete Written Solution
Problem Details

Below is a diagram of the DNA product of a PCR amplification of the region of the human genome containing p53. The white region represents the coding region of the gene. The letters above the DNA represent the locations of specific restriction enzyme cut sites. The numbers below each restriction site represent the number of kilobases (kb) from the first restriction site (at 0 kb). Each of the restriction enzymes creates unique sticky ends. 


The plasmid into which you want to clone the p53 gene is shown below.

After cutting the PCR product with HindIII and NotI, how many pieces of DNA will you have?

Frequently Asked Questions

What scientific concept do you need to know in order to solve this problem?

Our tutors have indicated that to solve this problem you will need to apply the DNA Cloning concept. You can view video lessons to learn DNA Cloning. Or if you need more DNA Cloning practice, you can also practice DNA Cloning practice problems.