Polymerase Chain Reaction - PCR Video Lessons

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Problem: Below is the sequence of the coding strand of DNA that flanks the coding region of the GFP gene:5'–ATCGGG–GFP gene–TTAGAC–3'Select the sequence of the forward primer that you would use to amplify the GFP gene using PCR. Remember that the forward primer hybridizes to the template strand of DNA. Select the sequence of the forward primer that you would use to amplify the GFP gene using PCR. Remember that the forward primer hybridizes to the template strand of DNA. A) 5'–GTCTAA–3' B) 5'–TAGCCC–3' C) 5'–ATCGGG–3' D) 5'–TTAGAC–3'

FREE Expert Solution

Recall:

Coding Strand: corresponds to the mRNA transcript

Template Strand: used to generate the mRNA transcript; complementary to the coding strand

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Problem Details

Below is the sequence of the coding strand of DNA that flanks the coding region of the GFP gene:

5'–ATCGGG–GFP gene–TTAGAC–3'


Select the sequence of the forward primer that you would use to amplify the GFP gene using PCR. Remember that the forward primer hybridizes to the template strand of DNA. Select the sequence of the forward primer that you would use to amplify the GFP gene using PCR. Remember that the forward primer hybridizes to the template strand of DNA. 


A) 5'–GTCTAA–3' 

B) 5'–TAGCCC–3' 

C) 5'–ATCGGG–3' 

D) 5'–TTAGAC–3'

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