Polymerase Chain Reaction - PCR Video Lessons

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Problem: Suppose you found the gel analysis results below. The letter at the top of each column is the abbreviation for the longer name written at the bottom right. If these were your results, what error likely occurred in your experiment? A. The gel was run for too long. Some ladder and PCR fragments ran off the gel. B. The gel was not run long enough for the samples to migrate from the wells. C. The positive control was contaminated, producing an incorrect result. D. More than one sample was loaded into the ladder well causing multiple bands.

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Agarose Gel Electrophoresis: used to analyze DNA and PCR products

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Problem Details

Suppose you found the gel analysis results below. The letter at the top of each column is the abbreviation for the longer name written at the bottom right. If these were your results, what error likely occurred in your experiment? 

A. The gel was run for too long. Some ladder and PCR fragments ran off the gel. 

B. The gel was not run long enough for the samples to migrate from the wells. 

C. The positive control was contaminated, producing an incorrect result. 

D. More than one sample was loaded into the ladder well causing multiple bands.

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What scientific concept do you need to know in order to solve this problem?

Our tutors have indicated that to solve this problem you will need to apply the Polymerase Chain Reaction - PCR concept. You can view video lessons to learn Polymerase Chain Reaction - PCR. Or if you need more Polymerase Chain Reaction - PCR practice, you can also practice Polymerase Chain Reaction - PCR practice problems.

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Based on our data, we think this problem is relevant for Professor Doctor's class at OLE MISS.