Polymerase Chain Reaction - PCR Video Lessons

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Problem: Which of the following is NOT one of the steps of the PCR cycle? A. The sample is heated to about 95 °C to separate the strands, so they can be replicated. B. The sample is cooled to about 50 °C, and the primers can bind to the single DNA strands. C. The sample is heated to 72 °C, and DNA polymerase synthesizes new copies of DNA. D. The sample is cooled to about 10 °C to deactivate the DNA polymerase until the next step is ready.

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Polymerase Chain Reaction: method of amplifying DNA; usually performed using Taq polymerase

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Problem Details

Which of the following is NOT one of the steps of the PCR cycle? 

A. The sample is heated to about 95 °C to separate the strands, so they can be replicated. 

B. The sample is cooled to about 50 °C, and the primers can bind to the single DNA strands. 

C. The sample is heated to 72 °C, and DNA polymerase synthesizes new copies of DNA. 

D. The sample is cooled to about 10 °C to deactivate the DNA polymerase until the next step is ready.

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