Polymerase Chain Reaction: method of amplifying DNA; usually performed using Taq polymerase
Which of the following is NOT one of the steps of the PCR cycle?
A. The sample is heated to about 95 °C to separate the strands, so they can be replicated.
B. The sample is cooled to about 50 °C, and the primers can bind to the single DNA strands.
C. The sample is heated to 72 °C, and DNA polymerase synthesizes new copies of DNA.
D. The sample is cooled to about 10 °C to deactivate the DNA polymerase until the next step is ready.
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