Problem: Peptides can be separated using an ion-exchange column based on their isoelectric (pl) values. At which pH values would two different peptides, one with a pl of 5.0 and the other with a pI of 9.4, bind to a caution- and anion-exchange column? Each peptide may be capable of binding to each column at more than one pH value.

FREE Expert Solution

Isoelectric Point: also known as pI; pH at which a peptide carries no net electrical charge or is electrically neutral

pH < pI: net charge is positive

pH = pI: net charge is zero

pH > pI: net charge is negative


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Problem Details

Peptides can be separated using an ion-exchange column based on their isoelectric (pl) values. At which pH values would two different peptides, one with a pl of 5.0 and the other with a pI of 9.4, bind to a caution- and anion-exchange column? Each peptide may be capable of binding to each column at more than one pH value.