Lethal Dose (LD50): the concentration required to kill 50% of test organisms
Place the steps in the correct order to reflect how a lethal dose may be determined.
A) Dilutions of virus are made to represent a range of concentrations.
B) Inoculated host cells are observed and the number of dead cells are counted.
C) The dilution which killed 50% of the tissue culture cells is identified.
D) Dilutions are inoculated into susceptible tissue cultures (or susceptible host organisms).
a. A D B C
b.D B C A
c. D A C B
d. A B D C
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