Below is a diagram of the DNA product of a PCR amplification of the region of the human genome containing p53. The white region represents the coding region of the gene. The letters above the DNA represent the locations of specific restriction enzyme cut sites. The numbers below each restriction site represent the number of kilobases (kb) from the first restriction site (0 kb). Each of the restriction enzymes creates unique sticky ends.
B = BamHI; E = EcoRI; H = HindIII; N = NotI; S = SmaI; X = XbaI
The plasmid into which you want to clone p53 gene is shown below.
Below is a diagram of the PCR product ligated into the plasmid. Drag the restriction enzyme names to the appropriate places on the plasmid diagram.
Frequently Asked Questions
What scientific concept do you need to know in order to solve this problem?
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