DNA Cloning Video Lessons

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Problem: Below is a diagram of the DNA product of a PCR amplification of the region of the human genome containing p53. The white region represents the coding region of the gene. The letters above the DNA represent the locations of specific restriction enzyme cut sites. The numbers below each restriction site represent the number of kilobases (kb) from the first restriction site (0 kb). Each of the restriction enzymes creates unique sticky ends.B = BamHI; E = EcoRI; H = HindIII; N = NotI; S = SmaI; X = XbaIThe plasmid into which you want to clone p53 gene is shown below.Below is a diagram of the PCR product ligated into the plasmid. Drag the restriction enzyme names to the appropriate places on the plasmid diagram.

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PCR Product: 


Plasmid:

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Problem Details

Below is a diagram of the DNA product of a PCR amplification of the region of the human genome containing p53. The white region represents the coding region of the gene. The letters above the DNA represent the locations of specific restriction enzyme cut sites. The numbers below each restriction site represent the number of kilobases (kb) from the first restriction site (0 kb). Each of the restriction enzymes creates unique sticky ends.

B = BamHI; E = EcoRI; H = HindIII; N = NotI; S = SmaI; X = XbaI


The plasmid into which you want to clone p53 gene is shown below.


Below is a diagram of the PCR product ligated into the plasmid. Drag the restriction enzyme names to the appropriate places on the plasmid diagram.

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