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Problem: The following data were obtained from partial cleavage and analysis of a pentadecapeptide: CNBr digestion peptides (not in order): CNBr 1: Ala, Gly, Leu, Lys, Met, ProCNBr 2: Ala, Arg, Asp, Cys, GIn, GlyCNBr 3: Met, Phe, Trp Trypsin digestion peptides (not in order): T1: Ala, Cys, GInT2: Ala, Gly, Lys, Met, Phe, TrpT3: Arg, Asp, Gly, Leu, Met, Pro N-terminus: Phe (for the pentadecapeptide)Gly (for CNBr1)Asp (for CNBr2)Cys (for T1)Leu (for T3) C-terminus: GIn Determine the sequence of the pentadecapeptide. Use three-letter abbreviations in your answer. Use dashes (-) between each abbreviation.

FREE Expert Solution

CNBr: cyanogen bromide; cuts from the carboxyl side of methionine

CNBr 1: Ala, Gly, Leu, Lys, Met, Pro

CNBr 2: Ala, Arg, Asp, Cys, GIn, Gly

CNBr 3: Met, Phe, Trp 


Trypsin: enzyme; cuts from the carboxyl side of lysine and arginine

T1: Ala, Cys, GIn
T2: Ala, Gly, Lys, Met, Phe, Trp
T3: Arg, Asp, Gly, Leu, Met, Pro 

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Problem Details

The following data were obtained from partial cleavage and analysis of a pentadecapeptide: 

CNBr digestion peptides (not in order): 

CNBr 1: Ala, Gly, Leu, Lys, Met, Pro
CNBr 2: Ala, Arg, Asp, Cys, GIn, Gly
CNBr 3: Met, Phe, Trp 

Trypsin digestion peptides (not in order): 

T1: Ala, Cys, GIn
T2: Ala, Gly, Lys, Met, Phe, Trp
T3: Arg, Asp, Gly, Leu, Met, Pro 

N-terminus: 

Phe (for the pentadecapeptide)
Gly (for CNBr1)
Asp (for CNBr2)
Cys (for T1)
Leu (for T3) 

C-terminus: GIn

Determine the sequence of the pentadecapeptide. Use three-letter abbreviations in your answer. Use dashes (-) between each abbreviation.

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