Enzyme Inhibitors

How might you determine whether inhibition is reversible or irreversible? Drag the terms on the left to the appropriate blanks on the right to complete the sentences.• charge• if not• activator• symmetry• nitrogen atoms• if so• inhibitor • covalently• halogens• heavy metals• non-covalentlyBecause irreversible inhibitors bind to the enzyme _______, you could analyze the enzyme molecule after adding the ______ to see if there was an increase of enzyme molecular weight or _________. You could test whether the inhibitor contains any _______, which often are irreversible inhibitors. You could test whether it is possible to separate the inhibitor from the inhibited enzyme (by dilution, for example) - _________, the inhibitor is irreversible.

A competitive inhibitorA. does not inhibit enzyme activity but does lower substrate concentration.B. binds at a site other than the active site.C. irreversibly binds and inactivates the enzyme.D. binds to and inactivates the substrate.E. cannot be processed by the enzyme.

if you find the inhibition is reversible, how would you determine whether the inhibition is competitive or noncompetitive? Drag the terms on the left to the appropriate blanks on the right to complete the sentences.• large• noncompetitive inhibition• competitive inhibition• decreases• increases• does not change• small If adding a ________ amount of substrate overcomes the inhibition and restores maximum enzyme activity, it is ________ (vmax _______, Km _______). If this does not restore enzyme activity, the inhibitor is binding at a separate site and is showing ________ (Km is not affected, vmax _______).