DNA Transcription

During transcription elongation, what is the function of DNA topoisomerases?A. identifying the region where termination will be taking place so that RNA polymerase can slow elongation before stoppingB. opening the DNA to form the transcription bubble for RNA polymerase to beginC. coiling the DNA after RNA polymerase has passed by so that it is ready for the next round of transcriptionD. removing positive DNA supercoils that are being produced by RNA polymeraseE. removing negative supercoiling so that the promoter region is available for sigma factor

Match each event with the appropriate stage of transcription

Identify the functions of an enhancer in transcription. a) A cis-acting regulatory sequence of DNA that reduces levels of transcription. b) A cis-regulatory element that regulates gene transcription in specific tissues or cells. c) Begins gene expression by disabling repressors and binding to the repressor elements. d) Inhibits transcription by binding to the operator. e) Regulates transcription by catalyzing the formation of an enhanceosome, activating transcription.

1. Which of the following are present in your liver cells?a. enhancers of genes expressed in the liverb. promoters of genes expressed in the liverc. enhancers of genes expressed in the kidneyd. promoters of genes expressed in the kidneye. All of the above are present in liver cells2. Why are genes for rRNA and tRNA considered to be genes even though they do not produce polypeptides?a. rRNA and tRNA are also gene products along with polypeptides.b. The structures of tRNA and rRNA are similar to the structures of polypeptides.c. The functions of rRNA and tRNA are similar to the functions of polypeptides.d. The statement is false; DNA sequences encoding for rRNA and tRNA are not actually genes.

 (T or F) TFIIH is a general transcription factor for eukaryotic RNA polymerase II.

Part A. Identify the following elements on a diagram of a transcription bubble. Drag the labels to their appropriate locations in the diagram. Labels may be used more than once.Part B. What is the direction of transcription in this diagram?A) From right to leftB) Unable to be determinedC) From left to rightPart C. Where would you expect to find the promoter for this gene?A) Right SideB) Left SideC) Unable to be determined

Which of the following is the enzyme complex that catalyzes transcription in bacterial cells?A. protein-dependent DNA polymeraseB. RNA-dependent DNA polymeraseC. DNA-dependent RNA polymeraseD. DNA-dependent DNA polymeraseE. protein-dependent RNA polymeraseF. RNA-dependent RNA polymerase

(T or F) In eukaryotic cells, transcription of ribosomal RNA is primarily the function of RNA polymerase II.