DNA Replication

An Okazaki fragment has which of the following arrangements? A) primase, polymerase, ligase B) 3' RNA nucleotides, DNA nucleotides 5' C) 5' RNA nucleotides, DNA nucleotides 3' D) DNA polymerase I, DNA polymerase III

Which enzyme catalyzes the elongation of a DNA strand in the 5' → 3' direction? A) primase B) DNA ligase C) DNA polymerase IIID) topoisomerase

What is the function of DNA polymerase III? A) to unwind the DNA helix during replication B) to seal together the broken ends of DNA strands C) to add nucleotides to the 3' end of a growing DNA strand D) to degrade damaged DNA molecules

The leading and the lagging strands of DNA formed during DNA replication differ in that A) the leading strand is synthesized in the same direction as the movement of the replication fork, and the lagging strand is synthesized in the opposite direction. B) the leading strand is synthesized by adding nucleotides to the 3' end of the growing strand, and the lagging strand is synthesized by adding nucleotides to the 5' end. C) the lagging strand is synthesized continuously, whereas the leading strand is synthesized in short fragments that are ultimately stitched together D) the leading strand is synthesized at twice the rate of the lagging strand.

A new DNA strand elongates only in the 5 to 3' direction because A) DNA polymerase begins adding nucleotides at the 5' end of the template. B) Okazaki fragments prevent elongation in the 3' to 5' direction. C) replication must progress toward the replication fork. D) DNA polymerase can only add nucleotides to the free 3' end.

Label the figure to assess your knowledge of DNA replication. 

If SSB is not present during DNA replication, what would you expect to see? A. SSB carries the helicase protein to the open region of DNA, so hydrolysis and strand separation will not occur. B. Helicase activity is inhibited, so DNA strands cannot be separated. C. SSB prevents reannealing of the separated strands, so strands would quickly reanneal and DNA replication cannot proceed. D. The replisome complex would not assemble on the oriC region. E. The DNA cannot bend, so hydrogen bonds in the 13-mer region of oriC remain intact.