Ch. 18 - BiotechnologyWorksheetSee all chapters
All Chapters
Ch. 1 - Introduction to Biology
Ch. 2 - Chemistry
Ch. 3 - Water
Ch. 4 - Biomolecules
Ch. 5 - Cell Components
Ch. 6 - The Membrane
Ch. 7 - Energy and Metabolism
Ch. 8 - Respiration
Ch. 9 - Photosynthesis
Ch. 10 - Cell Signaling
Ch. 11 - Cell Division
Ch. 12 - Meiosis
Ch. 13 - Mendelian Genetics
Ch. 14 - DNA Synthesis
Ch. 15 - Gene Expression
Ch. 16 - Regulation of Expression
Ch. 17 - Viruses
Ch. 18 - Biotechnology
Ch. 19 - Genomics
Ch. 20 - Development
Ch. 21 - Evolution by Natural Selection
Ch. 22 - Evolution of Populations
Ch. 23 - Speciation
Ch. 24 - History of Life on Earth
Ch. 25 - Phylogeny
Ch. 26 - Prokaryotes
Ch. 28 - Protists
Ch. 29 - Plants
Ch. 30 - Fungi
Ch. 31 - Overview of Animals
Ch. 32 - Invertebrates
Ch. 33 - Vertebrates
Ch. 34 - Plant Anatomy
Ch. 35 - Vascular Plant Transport
Ch. 36 - Soil
Ch. 37 - Plant Reproduction
Ch. 38 - Plant Sensation and Response
Ch. 39 - Animal Form and Function
Ch. 40 - Digestive System
Ch. 41 - Circulatory System
Ch. 42 - Immune System
Ch. 43 - Osmoregulation and Excretion
Ch. 44 - Endocrine System
Ch. 45 - Animal Reproduction
Ch. 46 - Nervous System
Ch. 47 - Sensory Systems
Ch. 48 - Muscle Systems
Ch. 49 - Ecology
Ch. 50 - Animal Behavior
Ch. 51 - Population Ecology
Ch. 52 - Community Ecology
Ch. 53 - Ecosystems
Ch. 54 - Conservation Biology
Sections
Introduction to DNA-Based Technology
Introduction to DNA Cloning
Steps to DNA Cloning
Introduction to Polymerase Chain Reaction
The Steps of PCR
Gel Electrophoresis
Southern Blotting
DNA Fingerprinting
Introduction to DNA Sequencing
Dideoxy Sequencing

Concept #1: Steps to DNA Cloning

Concept #2: Step 1) Create Recombinant DNA

Practice: Human DNA cut with restriction enzyme A can be joined to:

Concept #3: 1a) Use Restriction Enzymes

Practice: What is the enzymatic function of restriction enzymes?

Practice: Each restriction enzyme has a specific sequence of nucleotides where it cuts the DNA. These sequences of DNA are unique to each restriction enzyme and are known as:

Concept #4: 1b) Use Ligation Enzymes

Practice: The single-stranded ends of DNA molecules can be joined together by:

Concept #5: 2) Transform Recombinant DNA into Bacteria

Practice: The process of using DNA from one organism to alter the characteristics of another is called:

Practice: An organism which has foreign genes incorporated into its genomes is known as a:

Concept #6: Review & Application of DNA Cloning in Medicine

Practice: What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
I. Transform bacteria with a recombinant DNA molecule.
II. Cut the plasmid DNA using restriction enzymes (endonucleases).
III. Extract plasmid DNA from bacterial cells.
IV. Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments. V. Use ligase to seal plasmid DNA to non-plasmid DNA.