Ch. 18 - BiotechnologyWorksheetSee all chapters
All Chapters
Ch. 1 - Introduction to Biology
Ch. 2 - Chemistry
Ch. 3 - Water
Ch. 4 - Biomolecules
Ch. 5 - Cell Components
Ch. 6 - The Membrane
Ch. 7 - Energy and Metabolism
Ch. 8 - Respiration
Ch. 9 - Photosynthesis
Ch. 10 - Cell Signaling
Ch. 11 - Cell Division
Ch. 12 - Meiosis
Ch. 13 - Mendelian Genetics
Ch. 14 - DNA Synthesis
Ch. 15 - Gene Expression
Ch. 16 - Regulation of Expression
Ch. 17 - Viruses
Ch. 18 - Biotechnology
Ch. 19 - Genomics
Ch. 20 - Development
Ch. 21 - Evolution by Natural Selection
Ch. 22 - Evolution of Populations
Ch. 23 - Speciation
Ch. 24 - History of Life on Earth
Ch. 25 - Phylogeny
Ch. 26 - Prokaryotes
Ch. 28 - Protists
Ch. 29 - Plants
Ch. 30 - Fungi
Ch. 31 - Overview of Animals
Ch. 32 - Invertebrates
Ch. 33 - Vertebrates
Ch. 34 - Plant Anatomy
Ch. 35 - Vascular Plant Transport
Ch. 36 - Soil
Ch. 37 - Plant Reproduction
Ch. 38 - Plant Sensation and Response
Ch. 39 - Animal Form and Function
Ch. 40 - Digestive System
Ch. 41 - Circulatory System
Ch. 42 - Immune System
Ch. 43 - Osmoregulation and Excretion
Ch. 44 - Endocrine System
Ch. 45 - Animal Reproduction
Ch. 46 - Nervous System
Ch. 47 - Sensory Systems
Ch. 48 - Muscle Systems
Ch. 49 - Ecology
Ch. 50 - Animal Behavior
Ch. 51 - Population Ecology
Ch. 52 - Community Ecology
Ch. 53 - Ecosystems
Ch. 54 - Conservation Biology
Sections
Introduction to DNA-Based Technology
Introduction to DNA Cloning
Steps to DNA Cloning
Introduction to Polymerase Chain Reaction
The Steps of PCR
Gel Electrophoresis
Southern Blotting
DNA Fingerprinting
Introduction to DNA Sequencing
Dideoxy Sequencing

Concept #1: Southern Blotting

Practice: A southern blotting technique is used to detect a specific ______ sequence from a blood or tissue sample.

Practice: A _____ is a single-stranded DNA molecule used in hybridization reactions to detect the presence of a particular gene in an assortment of DNA fragments.
(Hybridization Reactions: reactions that combine two complementary single-stranded DNA molecules)

Concept #2: Steps of Southern Blotting

Practice: A geneticist wants to see if her patient has Gene X. The geneticist takes a blood sample from her patient and prepares a southern blot. How will the geneticist know if her patient possesses Gene X?

Practice: Place the following steps of Southern Blotting in the correct order.
a) ______: Filter paper is incubated with the labeled DNA probe which anneals to the ssDNA fragments.
b) ______: Analyze gel to determine the presence of the DNA sequence of interest.
c) ______: Separate DNA fragments by size using gel electrophoresis.
d) ______: Denature DNA by soaking gel in a basic solution.
e) ______: Fragment unknown DNA sample(s) using restriction enzymes.

Practice: By analyzing the Southern Blot results below, which of the samples contains the gene of interest?