What is common between miRNA and siRNA?
Describe how RNA differs from DNA in structure and function. List the three types of-RNA involved in protein synthesis and describe the function of each.
Name three post transcriptional modifications that are made to a RNA transcript in eukaryotes.
What is the driving force of chain growth/elongation in RNA synthesis? And explain how.
What are the differences and the similarities between transposable elements that utilize an RNA intermediate and those that do not? Be sure to cover structural, functional, and behavioral differences.
What is meant by A, P and E in the ribosome?
What causes the tRNA molecule to form stem-loops?
The following eukaryotic structural gene contains two introns and three exons.
The table below shows four possible mRNA products of this gene. Use the labels to explain what may have caused each mRNA. Drag the correct label to each location in the table. Labels may be used once, more than once, or not at all.
Which of the following is a characteristic of RNA splicing in Eukaryotes?
a. It involves recognition of sequence-specific intron/exon boundary sites by cytoplasmic proteins.
b. Exon/intron boundaries are typically characterized by a 5' GU splice junction and a 3' AG splice junction.
c. It involves removal of introns from a gene sequence followed by transcription and subsequent splicing of exons.
d. After splicing occurs, the U1, U2, U5, U6 snRNP complex removes remaining exons for degradation.
In Eukaryotes, what are the 3 modifications between the initial transcription of a premRNA and the time it leaves the nucleus. What are they?
Briefly explain the differences among messenger RNA, transfer RNA and ribosomal RNA in terms of the roles they play in transcription and translation and where they are found in the cell.
Describe the three stages of transcription: initiation, elongation, and termination.
For RNA transcription, define what determines where it begins, when it begins, and where it ends.
Construct a table listing the three types of RNA and for each write a paragraph describing its function and whether it deals with the process of transcription or translation.
If you compared the DNA sequence of a gene with the sequence of the mature mRNA that was transcribed from the gene you would find:
a. The mRNA is shorter because it does not contain exons
b. Both are the same length
c. The mRNA is shorter because it does not contain introns
d. The mRNA is shorter because each codon of three bases encodes only one amino acid
e. The mRNA is longer because each codon of one amino acid encodes three bases
In eukaryotic cells sequences of mRNA that are removed from an mRNA molecule before being translated are:
Introns are removed during RNA processing.