Concept: Proofreading and Repair Mechanisms7m
Concept: Telomeres and Telomerase7m
Consider the following DNA sequence:
5' - TAAGCTGCTG - 3'
3' - ATTCGACGAC - 5'
A. Deamination occurs at the C at position 7 (bottom strand). Show the double-stranded DNA daughter sequences following a round of DNA synthesis.
B. What would happen if the original sequence were irradiated with UV?
C. What repair pathway would be used to repair the mutation in (A)?
D. What repair pathway would be used to repair the UV products in (B)?
What is the composition of a telomerase enzyme
What are telomeres-where it is located?
In the synthesis of DNA ends by telomerase, what is the template strand, the end added, and the type of polymerase?
A. Lagging template strand, 5' end, DNA polymerase
B. Lagging template strand, 3' end, DNA polymerase
C. Leading template strand, 5' end, DNA polymerase
D. Leading template strand, 3' end, reverse transcriptase
E. Lagging template strand, 3' end, reverse transcriptase
Telomeres are sequences added to the ends of chromosome through the activity of telomerase, which contains reverse transcriptase (RT). Why is RT a DNA polymerase? What template does it use? What is the primer?