Biology Biology Practice Problems Solutions Library

PCR and Dideoxy Sequencing Solutions Library

Access 27 PCR and Dideoxy Sequencing video and text solutions to help you complete your homework. Need to revisit the concept? Watch our PCR and Dideoxy Sequencing learn videos.

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27 solutions

PCR and Dideoxy Sequencing

Q. Why is an enzyme from a thermophilic bacterium used in PCR?A. DNA is replicated at a high temperature that denatures most proteins.B. The enzyme ma...

Solved • Sep 27, 2018

PCR and Dideoxy Sequencing

Q. Which of the following is not required for PCR?A. DNA polymeraseB. dNTPSC. HelicaseD. PrimersE. None of the above (all are required)

Solved • Sep 25, 2018

PCR and Dideoxy Sequencing

Q. You are interested in the sequence of a 10-base segment f a gene. Using the chain-terminator procedure, you obtain the results shown below after ge...

Solved • Sep 23, 2018

PCR and Dideoxy Sequencing

Q. What is thesequence of the TEMPLATE strand synthesized using Sanger sequencing in the figure shown below? Report this sequence in the customary 5' ...

Solved • Sep 12, 2018

PCR and Dideoxy Sequencing

Q. PCR is a molecular biology technique that allows us to replicate DNA in an in vitro system without the need for a 'replisome'. What conditions or e...

Solved • Aug 31, 2018

PCR and Dideoxy Sequencing

Q. In a PCR reaction, if you start with a single copy of a DNA template, how many copies will there be after:A. 4 rounds of PCR amplification?B. 25 ro...

Solved • Aug 29, 2018

PCR and Dideoxy Sequencing

Q. Complementary DNA (cDNA) is a double-stranded molecule. In the laboratory, how is cDNA generated from a eukaryotic messenger RNA (mRNA)?A. Reverse ...

Solved • Aug 26, 2018

PCR and Dideoxy Sequencing

Q. Which statement about PCR is INCORRECT?A. is a way to duplicate DNA outside of an organismB. involves increasing the temperature of the DNA to caus...

Solved • Aug 24, 2018

PCR and Dideoxy Sequencing

Q. Why are you performing two PCR reactions on each DNA sample?

Solved • Jul 17, 2018

PCR and Dideoxy Sequencing

Q. Describe what DNA sequencing is

Solved • Jul 13, 2018

PCR and Dideoxy Sequencing

Q. A DNA fragment was sequenced using dideoxy DNA sequencing. In this procedure, ddATP was tagged with a green dye, ddCTP was tagged with a blue dye, ...

Solved • Jul 5, 2018

PCR and Dideoxy Sequencing

Q. In order to determine if a mutation has occured in Gene X, DNA is sequenced using the Sanger method. Briefly explain how this method works and then...

Solved • Jul 3, 2018

PCR and Dideoxy Sequencing

Q. A 13-nucleotide long DNA template was sequenced by the chain-terminator method (the Sanger method) using a 5'-TAG primer. The resulting fragments w...

Solved • Jul 2, 2018

PCR and Dideoxy Sequencing

Q. List the three steps in a PCR, and briefly indicate what happens at each step. 

Solved • Jun 25, 2018

PCR and Dideoxy Sequencing

Q. Polymerase chain reaction is known for its power of amplifying a target DNA sequence at a high speed. Each cycle can double the number of DNA molec...

Solved • Jun 7, 2018

PCR and Dideoxy Sequencing

Q. In the shotgun approach to whole-genome sequencing (shotgun sequencing), random DNA fragments of a chromosome are sequenced. The fragment sequences...

Solved • Jun 7, 2018

PCR and Dideoxy Sequencing

Q. In Northern blotting, electrophoresis is used to resolve which biological molecules? What type of probe is used to identify the target molecule(s)?

Solved • May 16, 2018

PCR and Dideoxy Sequencing

Q. What is the important feature of DNA polymerases used for PCR that distinguishes them from all other DNA polymerases?

Solved • May 9, 2018

PCR and Dideoxy Sequencing

Q. How will larger pieces of DNA move compared to smaller pieces of DNA?

Solved • Apr 30, 2018

PCR and Dideoxy Sequencing

Q. During the PCR, the hydrogen bonds of the double-stranded DNA molecules are broken by the enzyme helicase.a. Trueb. False

Solved • Oct 10, 2017

PCR and Dideoxy Sequencing

Q. PCR can be used to amplify DNA from any source.a. Trueb. False

Solved • Oct 10, 2017

PCR and Dideoxy Sequencing

Q. The most likely source of the Taq polymerase used in PCR is a bacterium that lives ina. soil.b. arctic ice.c. hot vents.d. humans.e. plants.

Solved • Oct 10, 2017

PCR and Dideoxy Sequencing

Q. If you start with one double-stranded DNA molecule and you perform SIX cycles of PCR, how many double-stranded copies of the DNA will you have?a. 6...

Solved • Oct 10, 2017

PCR and Dideoxy Sequencing

Q. PCR requires all of the following EXCEPTa. primers.b. DNA ligase.c. DNA polymerase.d. DNA of interest.e. deoxyrobinucleotides.

Solved • Oct 10, 2017

PCR and Dideoxy Sequencing

Q. PCR is used to _____.a. amplify a single gene or smaller piece of DNAb. create DNA without intronsc. insert foreign DNA into a vectord. All of these

Solved • Oct 10, 2017

PCR and Dideoxy Sequencing

Q. For the polymerase chain reaction (PCR) to work, the Taq _______ must be heat stable to avoid denaturation.  a. DNA polymerase b. RNA polymerase ...

Solved • Jul 31, 2017

PCR and Dideoxy Sequencing

Q. The polymerase chain reaction: a. Doubles a region of DNA each cycleb. Makes a single copy of a region of DNAc. Is used to determine the sequence o...

Solved • Jul 31, 2017