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DNA Cloning Solutions Library

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24 solutions

DNA Cloning

Q. You would like to clone the DNA of interest into the pDiddy cloning plasmid. You have the following restriction map of the region that includes the...

Solved • Oct 9, 2018

DNA Cloning

Q. What basic structural property of DNA serves as a basis for most techniques used in genetic engineering?A. gene sequencesB. nucleic acid hybridizat...

Solved • Oct 4, 2018

DNA Cloning

Q. The process of using DNA from one bacterium to alter the characteristics of another is called:A. translationB. transductionC. transcriptionD. trans...

Solved • Sep 27, 2018

DNA Cloning

Q. During the procedure to form recombinant DNA molecules, the purpose of the cell transformation step is toA. use the transformed cells to combine ve...

Solved • Sep 2, 2018

DNA Cloning

Q. Name the restriction endonuclease that cuts at the following sites. Indicate whether it cuts in a blunt or staggered manner.A. GAATTCB. CTCGAGC. TA...

Solved • Aug 21, 2018

DNA Cloning

Q. What is recombinant DNA technology? What are the safety issues related to recombinant DNA technology?

Solved • Aug 10, 2018

DNA Cloning

Q. You digest DNA from the frog Xenopus laevis with HindIII that produces sticky DNA ends. You can ligate your DNA to : (check all that apply)A. HindI...

Solved • Aug 10, 2018

DNA Cloning

Q. Which ofthe following is a palindromic restriction site?A. 5' - ACGGCA - 3'B. 5' - TGCGCC - 3'C. 5' - AAGGTT - 3'D. 5' - ACGCGT - 3'E. 5' - GCAGCA ...

Solved • Aug 9, 2018

DNA Cloning

Q. Jason is setting up a reaction with the restriction endonuclease EcoRI. The concentration of the EcoRI enzyme solution is 20,000 units/mL. Jason mi...

Solved • Jul 19, 2018

DNA Cloning

Q. How is a gene for a particular protein inserted into a plasmid?

Solved • Jul 5, 2018

DNA Cloning

Q. What is a restriction enzyme? What role do they play in biotechnology experiments?

Solved • Jul 3, 2018

DNA Cloning

Q. What is Recombinant DNA?

Solved • May 18, 2018

DNA Cloning

Q. Restriction enzymes cut DNA at random sites.a.Trueb. False

Solved • Oct 10, 2017

DNA Cloning

Q. "Sticky ends" area. single-stranded DNA sequences that are generated by staggered cuts.b. double-stranded DNA sequences that are generated by stagg...

Solved • Oct 10, 2017

DNA Cloning

Q. The function of the enzyme ligase is toa. add new DNA to the plasmid.b. remove DNA from the plasmid.c. form covalent bonds between cloned gene and ...

Solved • Oct 10, 2017

DNA Cloning

Q. Arrange the following events in the proper sequence for gene cloning.1 = Incorporate gene into bacterial plasmid2 = Isolate DNA from organism conta...

Solved • Oct 10, 2017

DNA Cloning

Q. Restriction enzymes cut only at specific sites and therefore are not useful for genetic engineering.a. Trueb. False

Solved • Oct 10, 2017

DNA Cloning

Q. Human DNA cut with restriction enzyme A can be joined toa. human DNA cut with restriction enzyme B.b. human DNA that is uncut.c. bacterial DNA cut ...

Solved • Oct 10, 2017

DNA Cloning

Q. The single-stranded ends of DNA molecules can be joined together bya. restriction endonucleases.b. DNA ligase.c. DNA polymerase.d. primase.e. helic...

Solved • Oct 10, 2017

DNA Cloning

Q. The single-stranded but complementary ends of the two DNA molecules cleaved by the restriction enzyme _____.a. are called "sticky ends"b. can bind ...

Solved • Oct 10, 2017

DNA Cloning

Q. The foreign DNA is sealed into the opening created by the restriction enzyme by _____.a. DNA polymeraseb. DNA repair enzymesc. DNA ligased. helicase

Solved • Oct 10, 2017

DNA Cloning

Q. The plasmid DNA is cleaved by _____.a. DNA ligaseb. DNA polymerasec. a restriction enzymed. helicase 

Solved • Oct 10, 2017

DNA Cloning

Q. Small accessory rings of DNA are called _____.a. recombinant DNAb. plasmidsc. clonesd. transposons

Solved • Oct 10, 2017

DNA Cloning

Q. The process used to rapidly produce DNA sequences of interest is DNA sequencing.  a. True b. False

Solved • Jul 31, 2017