🤓 Based on our data, we think this question is relevant for Professor Sharan's class at University of Western Ontario.
In DNA cloning, the primary goal is to successfully insert the DNA of interest as a plasmid to a viable cell. This would then make the gene available to be induced for expression. In inserting the target DNA, the function of restriction enzymes is involved. These enzymes are endonucleases that cut the target DNA from the source strand and are the same endonucleases to cut the restriction sites with the same sequence so it can serve as the insertion point.
You would like to clone the DNA of interest into the pDiddy cloning plasmid. You have the following restriction map of the region that includes the DNA of interest and the plasmid (E = EcoRI, H = HindIII, X = XbaI, S = SphI, N = NotI)
Which restriction enzyme(s) would you choose to clone the DNA of interest into the cloning vector?
B. E and H
C. S and N
D. X and N
Frequently Asked Questions
What scientific concept do you need to know in order to solve this problem?
Our tutors have indicated that to solve this problem you will need to apply the DNA Cloning concept. You can view video lessons to learn DNA Cloning. Or if you need more DNA Cloning practice, you can also practice DNA Cloning practice problems.
What professor is this problem relevant for?
Based on our data, we think this problem is relevant for Professor Sharan's class at University of Western Ontario.