In DNA cloning, the primary goal is to successfully insert the DNA of interest as a plasmid to a viable cell. This would then make the gene available to be induced for expression. In inserting the target DNA, the function of restriction enzymes is involved. These enzymes are endonucleases that cut the target DNA from the source strand and are the same endonucleases to cut the restriction sites with the same sequence so it can serve as the insertion point.
You would like to clone the DNA of interest into the pDiddy cloning plasmid. You have the following restriction map of the region that includes the DNA of interest and the plasmid (E = EcoRI, H = HindIII, X = XbaI, S = SphI, N = NotI)
Which restriction enzyme(s) would you choose to clone the DNA of interest into the cloning vector?
B. E and H
C. S and N
D. X and N
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Based on our data, we think this problem is relevant for Professor Sharan's class at University of Western Ontario.