Polymerase Chain Reaction (PCR) is a method of creating multiple copies (i.e. amplifying) of a particular segment of DNA. This is a useful component in DNA cloning since it can provide multiple copies of the experimental sequence that can be used for further tests.
In PCR, a thermal cycler is used to shift through different temperature levels. These lead us to three major steps: denaturation, annealing, and extension. The PCR starts with the system reaching equilibrium then adjusted to denature, followed by the annealing step, and ends with the extension step.
Why is an enzyme from a thermophilic bacterium used in PCR?
A. DNA is replicated at a high temperature that denatures most proteins.
B. The enzyme makes DNA that is more similar to human DNA.
C. It is cheaper to obtain from live microorganisms than producing the enzyme in a lab.
D. This thermophile's enzyme will synthesize DNA.
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