PCR and Dideoxy Sequencing Video Lessons

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Problem: Which of the following is not required for PCR?A. DNA polymeraseB. dNTPSC. HelicaseD. PrimersE. None of the above (all are required)

FREE Expert Solution

Polymerase Chain Reaction (PCR) is a method of creating multiple copies (i.e. amplifying) of a particular segment of DNA. This is a useful component in DNA cloning since it can provide multiple copies of the experimental sequence that can be used for further tests. 

In PCR, a thermal cycler is used to shift through different temperature levels. These lead us to three major steps: denaturation, annealing, and extension. The PCR starts with the system reaching equilibrium then adjusted to denature, followed by the annealing step, and ends with the extension step.

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Problem Details

Which of the following is not required for PCR?

A. DNA polymerase

B. dNTPS

C. Helicase

D. Primers

E. None of the above (all are required)

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Based on our data, we think this problem is relevant for Professor Bagarova's class at University of Massachusetts Boston.