Polymerase Chain Reaction (PCR) is a method of creating multiple copies (i.e. amplifying) of a particular segment of DNA. This is a useful component in DNA cloning since it can provide multiple copies of the experimental sequence that can be used for further tests.
In PCR, a thermal cycler is used to shift through different temperature levels. These lead us to three major steps: denaturation, annealing, and extension. The PCR starts with the system reaching equilibrium then adjusted to denature, followed by the annealing step, and ends with the extension step.
Which of the following is not required for PCR?
A. DNA polymerase
E. None of the above (all are required)
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Based on our data, we think this problem is relevant for Professor Bagarova's class at University of Massachusetts Boston.