As stated, there are several pathways for gene regulation. These include both negative and positive control for the expression of the target gene. For the lac operon, the presence of the repressor prevents the expression of the target genes.
Gene expression from the lac operon can be controlled in many ways, including a robust negative regulation strategy. Select mutations that would reduce the effectiveness of this negative regulation and increase lac operon activity.
A. mutation that deletes the lacI gene
B. mutation of the -10 promoter sequence to more closely resemble the E.coli consensus sequence
C. mutation that increases the affinity of the operator regions for lactose repressor
D. mutation that reduces the affinity of the lactose repressor for lactose
E. mutation of the -10 promoter sequence to be less similar to the E. coli consensus sequence
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