The Sanger method, or the chain-terminator method determines the sequence of the template strand through the use of the DNA polymerase reaction with a small concentration of dideoxynucleoside triphosphates (ddNTPs). These ddNTPs attach to the elongating chain but cause a premature termination due to the lack of OH group in the 3' carbon. Subsequent electrophoresis would give the sequence provided by the differently sized fragments produced in the reactions.
You are interested in the sequence of a 10-base segment f a gene. Using the chain-terminator procedure, you obtain the results shown below after gel electrophoresis.
A. What is the sequence read from the gel (5'→3')?
B. What is the sequence of the gene segments (5'→3')?
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