PCR and Dideoxy Sequencing Video Lessons

Video Thumbnail

Concept

Problem: You are interested in the sequence of a 10-base segment f a gene. Using the chain-terminator procedure, you obtain the results shown below after gel electrophoresis.A. What is the sequence read from the gel (5'→3')?B. What is the sequence of the gene segments (5'→3')?

FREE Expert Solution

The Sanger method, or the chain-terminator method determines the sequence of the template strand through the use of the DNA polymerase reaction with a small concentration of dideoxynucleoside triphosphates (ddNTPs). These ddNTPs attach to the elongating chain but cause a premature termination due to the lack of OH group in the 3' carbon. Subsequent electrophoresis would give the sequence provided by the differently sized fragments produced in the reactions.

View Complete Written Solution
Problem Details

You are interested in the sequence of a 10-base segment f a gene. Using the chain-terminator procedure, you obtain the results shown below after gel electrophoresis.

A. What is the sequence read from the gel (5'→3')?

B. What is the sequence of the gene segments (5'→3')?

Frequently Asked Questions

What scientific concept do you need to know in order to solve this problem?

Our tutors have indicated that to solve this problem you will need to apply the PCR and Dideoxy Sequencing concept. You can view video lessons to learn PCR and Dideoxy Sequencing. Or if you need more PCR and Dideoxy Sequencing practice, you can also practice PCR and Dideoxy Sequencing practice problems.