PCR and Dideoxy Sequencing Video Lessons

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Problem: What is thesequence of the TEMPLATE strand synthesized using Sanger sequencing in the figure shown below? Report this sequence in the customary 5' to 3' direction.

FREE Expert Solution

Sanger sequencing method is a sequencing method that employs chain termination. This utilizes the chain elongation of the DNA polymerase but causes premature termination in specific bases per well. This premature termination is due to the presence of dideoxynucleoside triphosphates (ddNTPs). They can serve as the bases for chain elongation but once they attach, their lack of 3' OH group makes it impossible for elongation to continue. This causes the formation of differently sized fragments, thus enables the determination of the sequence. 

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Problem Details

What is thesequence of the TEMPLATE strand synthesized using Sanger sequencing in the figure shown below? Report this sequence in the customary 5' to 3' direction.

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