Biology Practice Problems DNA Cloning Practice Problems Solution: During the procedure to form recombinant DNA molec...

Solution: During the procedure to form recombinant DNA molecules, the purpose of the cell transformation step is toA. use the transformed cells to combine vector and source DNA in order to form recombinant molecules.B. use the transformed cells as a source of DNA ligase to form recombinant molecules.C. use the transformed cells to cut the DNA at specific sites to generate fragments.D. all the aboveE. None of the above

Problem

During the procedure to form recombinant DNA molecules, the purpose of the cell transformation step is to

A. use the transformed cells to combine vector and source DNA in order to form recombinant molecules.

B. use the transformed cells as a source of DNA ligase to form recombinant molecules.

C. use the transformed cells to cut the DNA at specific sites to generate fragments.

D. all the above

E. None of the above

Solution

DNA transformation is the transfer of genetic information from the source to the target sequence through direct cellular uptake and integration. Through this, a gene for the expression of a product may be transferred to a bacteria that may then be induced to express the gene in question. This is a common method useful in DNA cloning.

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