Polymerase chain reaction (PCR) is a technique of amplifying (creating multiple copies) a target DNA through a thermal cycler with the help of primers, DNA polymerase, and free nucleotides. Basically, this in vitro process simulates the replication process with the help of different agents/factors.
PCR is a molecular biology technique that allows us to replicate DNA in an in vitro system without the need for a 'replisome'. What conditions or elements used in PCR mimic the effects of the following parts in the replisome? Explain your answer in 1 or 2 sentences.
C. DNA pol III
Frequently Asked Questions
What scientific concept do you need to know in order to solve this problem?
Our tutors have indicated that to solve this problem you will need to apply the PCR and Dideoxy Sequencing concept. You can view video lessons to learn PCR and Dideoxy Sequencing. Or if you need more PCR and Dideoxy Sequencing practice, you can also practice PCR and Dideoxy Sequencing practice problems.