PCR and Dideoxy Sequencing Video Lessons

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Problem: PCR is a molecular biology technique that allows us to replicate DNA in an in vitro system without the need for a 'replisome'. What conditions or elements used in PCR mimic the effects of the following parts in the replisome? Explain your answer in 1 or 2 sentences.A. HelicaseB. PrimaseC. DNA pol III

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Polymerase chain reaction (PCR) is a technique of amplifying (creating multiple copies) a target DNA through a thermal cycler with the help of primers, DNA polymerase, and free nucleotides. Basically, this in vitro process simulates the replication process with the help of different agents/factors.

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Problem Details

PCR is a molecular biology technique that allows us to replicate DNA in an in vitro system without the need for a 'replisome'. What conditions or elements used in PCR mimic the effects of the following parts in the replisome? Explain your answer in 1 or 2 sentences.

A. Helicase

B. Primase

C. DNA pol III

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