Restriction fragment length polymorphisms (RFLP) is a known method in genetic analysis. It aims to identify individuals through detection of differences in DNA sequence by the presence of particular DNA fragments. DNA samples here are digested with specific restriction endonucleases. RFLP probes then hybridize with one or more of the DNA fragments after these were separated via gel electrophoresis. Since the probes are made of labeled DNA sequences, the electrophoretic profile will show a blotting pattern unique to the individual.
A gene probe that is generated by isolating mRNA and using the enzyme reverse transcriptase to produce a DNA copy is called a(n):
A. mRNA probe
B. cDNA probe.
C. synthesized probe
D. cloned probe
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