Restriction fragment length polymorphisms (RFLP) is a known method in genetic analysis. It aims to identify individuals through detection of differences in DNA sequence by the presence of particular DNA fragments. This is used as probes in DNA fingerprinting as different samples would be more likely to produce different fragments.
Please place the following steps of DNA fingerprinting by RFLP analysis in the correct sequence.
Rank the options below:
A. Fragmentation of the DNA using restriction enzymes that will cut the DNA at specific sites.
B. Amplification of the DNA sample to be test via polymerase chain reaction (PCR).
C. DNA gel electrophoresis to separate the bands by size.
D. Comparison of the DNA bands of the samples collected from different locations to determine whether they are from the same person.
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