PCR and Dideoxy Sequencing Video Lessons

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Problem: In a PCR reaction, if you start with a single copy of a DNA template, how many copies will there be after:A. 4 rounds of PCR amplification?B. 25 rounds of PCR amplification?

FREE Expert Solution

Polymerase chain reaction (PCR) is a technique of amplifying (creating multiple copies) a target DNA through a thermal cycler with the help of primers (forward and reverse), DNA polymerase, and free nucleotides. If one is to extract a specific sequence of interest first from a long DNA, the first few cycles would be used to create the sequence of interest as an isolated strand. 

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Problem Details

In a PCR reaction, if you start with a single copy of a DNA template, how many copies will there be after:

A. 4 rounds of PCR amplification?

B. 25 rounds of PCR amplification?

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