PCR and Dideoxy Sequencing Video Lessons

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Problem: Complementary DNA (cDNA) is a double-stranded molecule. In the laboratory, how is cDNA generated from a eukaryotic messenger RNA (mRNA)?A. Reverse transcriptase generates two single-stranded cDNAs from different mRNA molecules which hybridize to form double-stranded DNA.B. Reverse transcriptase generates a single-stranded cDNA and then uses that cDNA as a template to synthesize a complementary RNA strand.C. Reverse transcriptase generates a single-stranded cDNA and the DNA ligase synthesizes the complementary strand.D. Reverse transcriptase generates a single-stranded cDNA and then DNA polymerase synthesizes the complementary strand.

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Complementary DNA (cDNA) is the complementary DNA strand synthesized using the mRNA as template. These products are usually utilized in a reverse transcription PCR (RT PCR) since this method employs mRNA as the initial polymerase substrate. Though the mRNA originated from a double-stranded DNA, the cDNA is not an exact copy of DNA since the DNA still contains the sequence of introns. Since the mRNA already has its introns removed, the DNA copy of the mRNA would not have the sequence, as well.

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Problem Details

Complementary DNA (cDNA) is a double-stranded molecule. In the laboratory, how is cDNA generated from a eukaryotic messenger RNA (mRNA)?

A. Reverse transcriptase generates two single-stranded cDNAs from different mRNA molecules which hybridize to form double-stranded DNA.

B. Reverse transcriptase generates a single-stranded cDNA and then uses that cDNA as a template to synthesize a complementary RNA strand.

C. Reverse transcriptase generates a single-stranded cDNA and the DNA ligase synthesizes the complementary strand.

D. Reverse transcriptase generates a single-stranded cDNA and then DNA polymerase synthesizes the complementary strand.

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