Ch.20 - BiotechnologyWorksheetSee all chapters
All Chapters
Ch.1 - Introduction to Biology
Ch.2 - Chemistry
Ch.3 - Water
Ch.4 - Carbon
Ch.5 - Biological Molecules
Ch.6 - Cells
Ch.7 - The Membrane
Ch.8 - Energy and Metabolism
Ch.9 - Respiration
Ch.10 - Photosynthesis
Ch.11 - Cell Signaling
Ch.12 - Cell Division
Ch.13 - Meiosis
Ch.14 - Mendelian Genetics
Ch.15 - Chromosomal Theory of Inheritance
Ch.16 - DNA Synthesis
Ch.17 - Gene Expression
Ch.18 - Regulation of Expression
Ch.19 - Viruses
Ch.20 - Biotechnology
Ch.21 - Genomics
Ch.22 - Development
Ch.23 - Evolution by Natural Selection
Ch.24 - Evolution of Populations
Ch.25 - Speciation
Ch.26 - History of Life on Earth
Ch.27 - Phylogeny
Ch.28 - Prokaryotes
Ch.29 - Protists
Ch.30 - Plants
Ch.31 - Fungi
Ch.32 - Overview of Animals
Ch.33 - Invertebrates
Ch.34 - Vertebrates
Ch.35 - Plant Anatomy
Ch.36 - Vascular Plant Transport
Ch.37 - Soil
Ch.38 - Plant Reproduction
Ch.39 - Plant Sensation and Response
Ch.40 - Animal Form and Function
Ch.41 - Digestive System
Ch.42 - Circulatory System
Ch.43 - Immune System
Ch.44 - Osmoregulation and Excretion
Ch.45 - Endocrine System
Ch.46 - Animal Reproduction
Ch.47 - Nervous System
Ch.48 - Sensory Systems
Ch.49 - Muscle Systems
Ch.50 - Ecology
Ch.51 - Animal Behavior
Ch.52 - Population Ecology
Ch.53 - Community Ecology
Ch.54 - Ecosystems
Ch.55 - Conservation Biology
Problem

Complementary DNA (cDNA) is a double-stranded molecule. In the laboratory, how is cDNA generated from a eukaryotic messenger RNA (mRNA)?

A. Reverse transcriptase generates two single-stranded cDNAs from different mRNA molecules which hybridize to form double-stranded DNA.

B. Reverse transcriptase generates a single-stranded cDNA and then uses that cDNA as a template to synthesize a complementary RNA strand.

C. Reverse transcriptase generates a single-stranded cDNA and the DNA ligase synthesizes the complementary strand.

D. Reverse transcriptase generates a single-stranded cDNA and then DNA polymerase synthesizes the complementary strand.

Solution

Complementary DNA (cDNA) is the complementary DNA strand synthesized using the mRNA as template. These products are usually utilized in a reverse transcription PCR (RT PCR) since this method employs mRNA as the initial polymerase substrate. Though the mRNA originated from a double-stranded DNA, the cDNA is not an exact copy of DNA since the DNA still contains the sequence of introns. Since the mRNA already has its introns removed, the DNA copy of the mRNA would not have the sequence, as well.

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