PCR and Dideoxy Sequencing Video Lessons

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Problem: Which statement about PCR is INCORRECT?A. is a way to duplicate DNA outside of an organismB. involves increasing the temperature of the DNA to cause it to unwindC. requires a restriction enzymeD. requires DNA polymeraseE. does not use DNA helicase

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Polymerase Chain Reaction (PCR) is a method of creating multiple copies (i.e. amplifying) of a particular segment of DNA. This is a useful component in DNA cloning since it can provide multiple copies of the experimental sequence that can be used for further tests. 

In PCR, a thermal cycler is used to shift through different temperature levels. These lead us to three major steps: denaturation, annealing, and extension. The PCR starts with the system reaching equilibrium then adjusted to denature, followed by the annealing step, and ends with the extension step.

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Problem Details

Which statement about PCR is INCORRECT?

A. is a way to duplicate DNA outside of an organism

B. involves increasing the temperature of the DNA to cause it to unwind

C. requires a restriction enzyme

D. requires DNA polymerase

E. does not use DNA helicase

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