Polymerase Chain Reaction (PCR) is a method of creating multiple copies (i.e. amplifying) of a particular segment of DNA. This is a useful component in DNA cloning since it can provide multiple copies of the experimental sequence that can be used for further tests.
In PCR, a thermal cycler is used to shift through different temperature levels. These lead us to three major steps: denaturation, annealing, and extension. The PCR starts with the system reaching equilibrium then adjusted to denature, followed by the annealing step, and ends with the extension step.
Which statement about PCR is INCORRECT?
A. is a way to duplicate DNA outside of an organism
B. involves increasing the temperature of the DNA to cause it to unwind
C. requires a restriction enzyme
D. requires DNA polymerase
E. does not use DNA helicase
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