The translation process uses the mRNA synthesized in the nucleus as template to synthesize its polypeptide equivalent. For this problem, in order to determine the DNA sequence, the actual sequence of the mRNA must be known first.
You have synthesized the following protein: fMet-Pro-Asp-Gly-Thr. You accomplished this in a cell-free system containing tRNA molecules with the anticodons listed below
3' - CCG - 5'; 3' - UGU- 5'; 3' - GGC - 5'; 3' - UAC - 5'; 3' - CUG - 5'; mRNA
Construct the double-stranded DNA molecle from which this protein was synthesized. Show all your reasoning.
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